SRB

SRB Medium 10X (for making 500mL)

MgCl2 . 6H20			8.132g
NH4Cl				5.35g
K2HPO4 . 3 H20			2.51g
CaCl2 . 2H20			0.44g
lactate syrup			46.71mL
HEPES (not sodium HEPES)	32.5g
Na2SO4				14.2g

Filter sterilize


1X SRB Freshwater (for making 100mL)

10x SRB medium		10mL
trace elements		0.8mL
vitamins		0.1mL
yeast extract		0.1g

pH to 7.0 with NaOH
autoclave


1X SRB saltwater (for making 1L)

1x SRB freshwater medium	1L
NaCl				20g
MgCl2 . 6H20			1.4g

pH to 8.0
autoclave